Science Memes

10940 readers

1991 users here now

Welcome to c/science_memes @ Mander.xyz!

A place for majestic STEMLORD peacocking, as well as memes about the realities of working in a lab.

Rules

Don't throw mud. Behave like an intellectual and remember the human.
Keep it rooted (on topic).
No spam.
Infographics welcome, get schooled.

This is a science community. We use the Dawkins definition of meme.

Research Committee

[email protected]

Other Mander Communities

Science and Research

Biology and Life Sciences

Physical Sciences

Humanities and Social Sciences

Practical and Applied Sciences

Memes

Miscellaneous

founded 2 years ago

MODERATORS

[email protected]

417

Miracle cures (mander.xyz)

submitted 5 months ago by [email protected] to c/[email protected]

56 comments fedilink hide all child comments

you are viewing a single comment's thread
view the rest of the comments

[–] [email protected] 2 points 5 months ago (1 children)

did you unclog the column or does lab has to eat up few thousand dollar loss

[–] [email protected] 1 points 5 months ago (1 children)

We unclogged the column, but since it was clogging it up for each sample and it's quite damaging to the column, we decided to stop. Our budget was quite tight, since this was a school project.

[–] [email protected] 1 points 5 months ago (1 children)

jeez dude are you using some pretreatment

[–] [email protected] 1 points 5 months ago (1 children)

Idk what pretreatment really entails, but we did dilute it, if that counts 😅

[–] [email protected] 2 points 5 months ago

All HPLC samples that i do, before putting them on column i filter them through short layer of silica to separate all the insoluble and entirely too polar crap

Additionally there's also short guard column before main column, so if something goes really fucking wrong it's the 2cm guard column that is to be replaced and not the 20cm main one

I work mostly with NP-HPLC so your situation might be a bit different, but the general principle is there